The Three Lions face Santi Denia's side at the Fortuna Arena in Prague, aiming to bounce back from Saturday's 3-2 defeat against Spain at Wembley Stadium.
Hall, an unused substitute against the World champions, believes the Nations League is "a competition we'll look to try and win" and is aiming to give a good account of himself after missing out on Thomas Tuchel's World Cup squad last summer.
Speaking at Monday's pre-match media conference, the 22-year-old defender said: "It's interesting because all of the lads had been together for seven weeks in the summer, creating good bonds and relationships and understood what was required of them.
"For me, it's about coming in and indulging in that, taking on all the information and whenever I get my opportunity, make sure I do the right things.
"Whenever there's injuries or different things that happen, there's always opportunities for other players. There's been a lot of good left-backs over the last couple of years that have played in that role. Whenever I get my opportunity, I'm going to try and take it."
Hall has made a strong start to the current campaign at club level, scoring his first goal at St. James' Park in Newcastle United's 2-1 win against Hull City in the Premier League as well as signing a new long-term contract with his boyhood club.
The Slough-born full-back, capped four times by his country at senior level, is now aiming to use the frustrations of missing out on a first major international tournament of his career to his advantage when representing "one of the best countries in the world".
He added: "Whenever you've been in good form throughout the season, like I thought I was last year, anyone who is in a similar situation to me who didn't get selected is going to [feel] a sense of disappointment.
"I'm looking to use that to bounce back, and hopefully I can create a good account of myself.
"It's a heavy shirt. You're representing England so, whenever you step on the pitch, you've got to be at your best because there's a lot at stake.
"Everyone who is in the squad, and even players who aren't in the squad, know how much it means to each other to play for our country. There's always nice competition and it's healthy to get the best out of each other."
With the top two sides from Group A3 advancing into March's quarter-finals, Hall is determined to prevail against Czechia to enhance their bid of sealing a last-eight spot.
Hall is also relishing the prospect of going head-to-head with Magpies teammate Lukáš Horníček, with the Czech goalkeeper making his competitive debut for his country in last week's 2-1 defeat against Croatia.
"He's a great keeper and I know him well but we'll be looking to win the game," Hall said. "As much as he's a great guy, I hope he's not on it tomorrow!
"I'm expecting a really difficult game. We know they're a very proud nation and whenever you play international football, from what I've experienced so far, it's always tough games and looking forward to it."
